Archive for the ‘Microbiology’ Category

Of the German #Ebola Response Protocols

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SS: apparently the germans have different #Ebola protocols than the usa. and personally i am not convinced that those P95 or P100 clothe masks they are wearing is enough. regardless of whether i would wear a cloth mask or a full respirator mask is beside the point i am attempting to make here. according to german protocol they are getting hosed down by bleach before removing their contaminated #Ebola suits. everyone who has their #Ebola suits take note that you will require to bleach yourself down at the entrance of your home before removing your #ebola suits.

SS: i do not listen to anything obama says regarding catastrophes. obama’s catastrophe crisis track record is not very good. 1. we think he stop the oil leak in the gulf but we can’t really prove it. 2. obama stopped monitoring for fukushima radiation only one month after the accident and has done absolutely nothing except cover up fukushima radiation and the western coast. 3. the Louisiana methane sinkhole has been releasing massive amounts of methane into the atmosphere for two years and and obama pretends it doesnt exist. 

SS: my new book “Of the Obama Mahabharata Catastrophes” talks about these different mega-catastrophes. and… for the price of a burger and fries from mcdonalds i give some good advices how to cure yourselves. research science study showed us that if a person is able to remove radioactive particles from the body faster than accumulate them will drastically improve life span. and that if stop ingesting radiation that all the damage done can be nearly and even completely healed.

The sun shines behind chestnut leaves during warm and sunny autumn weather near Muensing south of Munich, Germany, Saturday, Oct. 12, 2014. (AP Photo/Matthias Schrader)

Volunteers of German forces Bundeswehr get sprayed bleach mixture on their protective suits during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

Volunteers of the German forces Bundeswehr get sprayed a bleach mixture on their protective suits during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

A volunteer of the German forces Bundeswehr gets sprayed a bleach mixture on his protective suit during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

A volunteer of the German forces Bundeswehr helps to fix a glove of a protective suit during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

A volunteer of the German forces Bundeswehr gets sprayed a bleach mixture on his protective glove during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

Volunteers of German forces Bundeswehr line up for decontamination of their protective suits during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

A volunteer of the German forces Bundeswehr gets sprayed a bleach mixture on his protective suit and gloves during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

Volunteers of the German forces Bundeswehr line up for a decontamination of their protective suits during a training for the Ebola virus in Appen about 25 kilometers (15,5 miles) north of Hamburg, Germany, Thursday, Oct. 23, 2014. The volunteers of the German forces Bundeswehr get trained for a mission to support the German Red Cross in Liberia in the fight against the Ebola epidemic. (AP Photo/Markus Schreiber)

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    Of the PH Dependent Ebola Glycoproteins Virus

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    SS: what is going on with #Ebola ?? the obama administration refuses, not just refuses but actually fights, to not halt flights from infected countries. this alone should be enough to clue us in that the obama admin and CDC tom friedman are doing the very minimal to contain this #ebola from spreading in the USA. Obama admin keeps repeating over and over that #Ebola is not like the flu that we cannot catch it from being airborne. obama admin claims it is instead like aids that can only be spread via bodily fluid contact, objects infected with ebola, or infected animals.

    SS: therefoere based upon what the obama admin is spreading throughout the land about #ebola the CDC has implemented the very minimal of protocols. nurses have been told they can just wear a gown, mask, and face shield… yet two nurses following these protocols are now sick with a %50 chance of survival and all tom friedman can tell us is about how something wasn’t done right, mistakes were made, and so sad to bad about the nurses being sick. that is the attitude. the two nurses are sick due to the obama admin and the CDC incompetence.. period.

    SS: i believe in doing things different. instead of doing the very minimum i think we should do more the maximum to protect americans, their families, and children. of course this makes to much sense for obama and his hosts of ebola zombies who follow him..  if we recall the obama admin used this same strategy as clouds of iodine-131 radiation flew across the USA. the obama admin told everyone to not use iodine that it would hurt americans. they lied. i personally have been taking iodine since the day of the fukushima catastrophe with no harmful side effects. listening to the obama admin is simply hazardous to our health.

    SS: it would be better for americans to take full precautions against #Ebola rather than obamas do the very minimal. in fact obamas policy of doing the very minimal could be considered negligence. the virus has spread to the usa because obama refused to halt flights from infected countries. it is a fact that the duncan fellow hopped onto an airplane and flew into Dallas and his arrival could have been prevented had obama halted flights. it is a fact that two nurses have gotten sick caring for duncan fellow using the CDC protocols.

    SS: Obama admins and tom friedmans attempts to finger point in the face of a catastrophe instead of halting flights and doing the maximum protocols proves they are at the least incompetent. Mistakes have already been made and they are going to continue to make mistakes because they are not trying to stop this virus. instead they are *ALLOWING* the virus to take hold and spread.

    SS: for regular readers of my blog i will tell you how to side step this #ebola problem. first the ebola virus infection has a much harder time attacking cells in a high alkaline ph body. this means that the very first thing a person should do are the things i have described in my new book “Of the Obama Mahabharata Catastrophes” to fight a fungus such as cancer caused by radiation poisoning. second, we need the ability to attack the virus, fungus, or bacteria. once again using the same suggestions in my book we do have the possibilities to attack and kill virus. the us government under obama has once again (like he told everyone not to use iodine during the fukushima clouds) told everyone not to use “colloidal silver”, yet it is well known to attack and kill virus, fungus, and bacteria. collodial silver has been shown in tests to inhibit virus that use glycoprotien binding the inability to bind to host cells. and of course organic vitamins, especially vitamin c. if have a strong immune system then you can be immune to #ebola.

    SS: i think should be prepared by obtaining ebola suits which include a respirators. this is not a halloween joke, instead this is real. take the #ebola threat serious. store food and water. add .999 silver coins to water to keep it fresh. cook all food and filter all water with carbon filters. i am keeping several bags of pool shock to use as a cleaning solutions like bleach.

    CDC: You Can Give—But Can’t Get—Ebola on a Bus October 15, 2014 “I think there are two different parts of that equation,” he continued. “The first is, if you’re a member of the traveling public and are healthy, should you be worried that you might have gotten it by sitting next to someone? And the answer is no.” “Second, if you are sick and you may have Ebola, should you get on a bus? And the answer to that is also no. You might become ill, you might have a problem that exposes someone around you,” he said.

    ‘Who’s the idiot with the clipboard?’ Disbelief and panic as mystery man WITHOUT a hazmat suit helps second Ebola nurse board her plane to Atlanta, disposes waste and then climbs aboard 15 October 2014 | As news helicopters swarmed over Dallas’ Love Field on Wednesday evening to watch the second U.S. nurse to contract Ebola board a private plane bound for Atlanta, one lone mysterious man stood out from the pack. Holding a clipboard and directing the transfer, the unidentified man seemed to be the only person on the tarmac without protective clothing, wearing just a button down shirt and slacks. While Ebola is not an airborne disease, his presence so close to patient Amber Vinson’s medical team sparked fears after he was seen grabbing a container and hazmat trash bag from one of the workers’ in full-protective gear and later boarding the flight. He then flew with Vinson and the other hazmat-suited medical staff to Atlanta and local television crews spotted him with the stricken nurse as she disembarked at the airport in Georgia to be transferred to Emory University Hospital. ABC News reports that the man is a supervisor for Phoenix Air, the company that flew Miss Vinson from Dallas to Atlanta. When the plane landed in Atlanta, the man had still not donned any protective clothing and was seen openly interacting with Vinson and the other medical professionals caring for the nurse. 

    Detection of cell-cell fusion mediated by Ebola virus glycoproteins.2006 Mar;80(6):2815-22.Ebola viruses (EboV) are enveloped RNA viruses infecting cells by a pH-dependent process mediated by viral glycoproteins (GP) involving endocytosis of virions and their routing into acidic endosomes. As with well-characterized pH-dependent viral entry proteins, in particular influenza virus hemagglutinin, it is thought that EboV GP require activation by low pH in order to mediate fusion of the viral envelope with the membrane of endosomes.

    Fusion of viral and cellular membranes is pH dependent. “The plasma membrane of eukaryotic cells serves as a barrier against invading parasites and viruses. To infect a cell, viruses must be capable of transporting their genome and accessory proteins into the host cell, bypassing or modifying the barrier properties imposed by the plasma membrane. Entry into the host cells always involves a step of membrane fusion for enveloped animal viruses. Other enveloped viruses such as orthomyxoviruses, alphaviruses or rhabdoviruses enter the cells by the endocytic pathway, and fusion depends on the acidification of the endosomal compartment. Fusion at the endosome level is triggered by conformational changes in viral glycoproteins induced by the low pH of this cellular compartment.”[1]

    In membrane biology, fusion is the process by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. The conformational transition occurs in a narrow pH range, corresponding to the optimal pH of fusion, in which the protein acquires the ability to interact with detergent micelles and lipid vesicles. This interaction leads to the insertion of the fusion peptide into the membrane, where a pore is formed. It has been suggested that the hepatitis C virus (HCV) infects host cells through a pH-dependent internalization mechanism. This HCVpp-mediated fusion was dependent on low pH, with a threshold of 6.3 and an optimum at about 5.5.[2]

    When pH drops to 6 or below, rapid fusion between the membranes of viruses and the liposomes occurs. This results in the transfer of viral nucleocapsids into the liposomes. Ebola virions are taken into endothelial cells via macropinocytosis. After their formation, macropinosomes move further into the cytoplasm to acquire new markers or fuse with other vesicles of the standard endolysosomal pathway. This eventually moves the Ebola virions to more acidic compartments such as early and late endosomes that assist in the pH dependent fusion of viral and cellular membranes.[3] During this process, the cell detaches from its neighbors and loses contact with its basement membrane thanks to a mechanism of glycan mediated steric occlusion by GP.[4] The newly created particles then leave via lipid rafts, leaving a destabilized vascular system responsible for the massive blood loss characteristic of Ebola patients.[5]

    Interaction of silver nanoparticles with HIV-1 29 June 2005 The regular spatial arrangement of the attached nanoparticles, the center-to-center distance between nanoparticles, and the fact that the exposed sulfur-bearing residues of the glycoprotein knobs would be attractive sites for nanoparticle interaction suggest that silver nanoparticles interact with the HIV-1 virus via preferential binding to the gp120 glycoprotein knobs. Due to this interaction, silver nanoparticles inhibit the virus from binding to host cells, as demonstrated in vitro.

    Silver found to increase effectiveness of antibiotics June 20, 2013 What they found was that even small amounts of silver ran roughshod over some of the toughest bacteria around. “It did two things,” Collins said. The positively charged silver ions degraded the bacteria’s protective layer, giving the antibiotics easier access to the pathogens’ innards. It also messed with the bugs’ metabolism and their ability to manage their iron levels.

    3rd Ebola patient, Amber Vinson, boards the plane that will take her to Atlanta.  Vinson will be admitted and treated at Emory University Hospital. (CBSDFW Screen Capture)

    Louisiana’s attorney general is trying to stop Dallas Ebola victim Thomas Eric Duncan’s incinerated belongings from going into a Louisiana landfill. Six truckloads of material were collected from Duncan’s apartment and incinerated at a facility in Port Arthur, Texas on Friday. The material, which included linen, bedding and carpet, was supposed to be shipped to a landfill in Carlyss, Louisiana for disposal. However, Attorney General James D. “Buddy” Caldwell said he will ask for a temporary restraining order to prevent that from happening. Caldwell said in a statement provided to Mashable, “We certainly share sadness and compassion for those who have lost their lives and loved ones to this terrible virus, but the health and safety of our Louisiana citizens is our top priority.” (Wochit Screen Capture)

    The second nurse to contract Ebola in the United States was receiving care at an Atlanta hospital on Thursday, a day after the news emerged that officials did not stop her flying on a commercial flight even after she reported a slight temperature. The U.S. Centers for Disease Control and Prevention said, the nurse, 29-year-old Amber Vinson, flew from Cleveland to Dallas on Monday, a day before she was diagnosed with Ebola. (Wochit Screen Capture)

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